Sodium Bicarbonate → HCID 178508521, compound

Participants

Reaction Steps

950 mg of 7β-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(R S)-(tert-butoxycarbonyl)(3,4-diacetoxyphenylthio)methoxy]imino]acetamido]-3-(1,2,3-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylate obtained from Example 22 was suspended in 30 ml of water, and saturated sodium hydrogen carbonate aqueous solution was added therein at the room temperature in order to make pH 8. After stirring for 2.5 hrs. at the room temperature, the aqueous solution was adsorbed on Diaion HP-20 and was eluted with water-methanol. The fraction containing the desired product was collected, concentrated and then, lyophilized to give 419 mg of disodium 7β-[(Z)-2-(2-amino-4-thiazolyl)-2-[[(R S)-(carboxy)(3,4-dihydroxyphenylthio)methoxy]imino]acetamido]-3-(1,2,3-thiadiazol-5-yl)thiomethyl-3-cephem-4-carboxylate.

Provenance & Evidence