HCID 67604151, compound → HCID 70063386, compound

Participants

Reaction Steps

Porcine liver esterase (200 μL, Sigma, 11 mg/mL in 3.2 M (NH4)2SO4 at pH=8) was added to the final product of Example 28 in 20mL of 0.1 M phosphate buffer (pH=7.4). After 20 h at 23° C., the reaction mixture was concentrated in vacuo. The residue was dissolved in 1N HCl (3 mL) and subsequently diluted with acetonitrile (5 mL) followed by immediate purification by reverse phase HPLC using the conditions of Example 1 to afford 23.0 mg (83%) of the title compound. The product was verified by 13C NMR (CD3OD) δ 1.8, 2.6, 15.0, 30.3, 31.8, 39.1, 50.6, 119.2, 122.1, 128.6, 144.3, 166.8, 172.0, 172.4, 173.4; Fast Atom Bombardment mass spectrometry (MH+)=347.

Provenance & Evidence