Phosphate → Phosphate Buffered Saline

HRID 2259551Notes

Reaction equation

[P:1]([O-:5])([OH:4])([OH:3])=[O:2].[K+:6].[P:7]([O-:11])([OH:10])([OH:9])=[O:8].[Na+:12].[Cl-:13].[Na+].P([O-])([O-])([O-])=O.[N-]=[N+]=[N-].[Na+]>>[OH:3][P:1]([O-:5])([OH:4])=[O:2].[OH:9][P:7]([O-:11])([O-:10])=[O:8].[Na+:12].[Na+:12].[Na+:12].[Cl-:13].[Cl-:13].[K+:6].[K+:6]

Participants

Reactant 5

HCID 1061Phosphate

HCID 5234Sodium Chloride2×

HCID 33557Sodium Azide

HCID 516951Monobasic potassium phosphate

HCID 23672064Monosodium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Procedure

A buffer was prepared from potassium dihydrogen phosphate, sodium dihydrogen phosphate (12 hydrate) and sodium chloride to make phosphate concentration of 0.036M, NaCl concentration of 0.156M and pH 6.5. To this buffer was added NaN3 at a concentration of 0.1% (w/v).

Sources & evidence

ORD record: ord-116b9c30024f4e6a9f517ed4b2bce459
Source dataset: uspto-grants-1995_12
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05474900

Reaction notes

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