HCID 135501905, compound → HCID 135493097, compound

Participants

Reaction Steps

Dissolve 1-amino-cyclopropanecarboxylic acid 2-(2-amino-6-oxo-1,6-dihydro-purin-9-ylmethoxy)-ethyl ester trifluoroacetic acid salt (0.02 g, 0.05 mmol) of Example 1 in 1 mL methanol. Apply this solution to a 1 g Varian Mega Bond Elute® SCX column (Varian Part# 170532) pre-washed several times with methanol. Wash the column with 10 mL of methanol then elute the compound with 15 mL of a 2 M solution of ammonia in methanol. Concentrate in vacuo to obtain the desired free base as a white solid. MS (ES+): 309.2 (M+1)

Provenance & Evidence