Ammonium Chloride → 1-(2-Chlorophenyl)-1H-pyrazolo[3,4-b]pyridin-6(7H)-one

Temperature

-35 °C

At −35° C., KHMDS (8.03 mL of 0.5 M in toluene, 4.01 mmol) was added to a slurry of methyl p,p-bis(2,2,2-trifluoroethyl)-phosphonoacetate (1.21 g, 3.82 mmol) and 18-crown-6 (506 mg, 1.91 mmol) in 3.0 mL of THF. After the resulting mixture was stirred at −35° C. for 10 min, a solution of 5-amino-1-(2-chlorophenyl)-1H-pyrazole-4-carbaldehyde (424 mg, 1.91 mmol) in THF (1.5 mL+1.5 mL rinsing) was added in via a syringe. The reaction mixture was slowly warmed to RT overnight, quenched with saturated NH4Cl, and extracted with EtOAc twice. The combined EtOAc layers were dried and concentrated to dryness. The resulting brown residue in THF (3.0 mL) at RT was treated with KHMDS (12 mL of 0.5 M in toluene, 6.0 mmol) and heated at 70° C. in an oil bath for 3 h. After the reaction mixture was cooled to RT, it was treated with saturated NH4Cl, and extracted with EtOAc twice. The combined EtOAc layers were dried and concentrated. The brown residue was loaded on an ISCO 12 g column (eluted with 25-65% EtOAc in Hexanes) to give the title compound (352 mg) as a yellow amorphous solid. MS (ESI, pos.ion) m/z: 246.0 (M+1).

1. temperatureThe reaction mixture was slowly warmed to RT overnight
2. customquenched with saturated NH4Cl
3. extractionextracted with EtOAc twice
4. dry with materialThe combined EtOAc layers were dried
5. concentrationconcentrated to dryness
6. temperatureheated at 70° C. in an oil bath for 3 h
7. temperatureAfter the reaction mixture was cooled to RT
8. extractionextracted with EtOAc twice
9. dry with materialThe combined EtOAc layers were dried
10. concentrationconcentrated
11. washThe brown residue was loaded on an ISCO 12 g column (eluted with 25-65% EtOAc in Hexanes)

ORD record

ord-a65a5c8597f44c8bb1f2dc1c23ec97ec

Source dataset

uspto-grants-2010_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US07846944B2