dCTP → Datp

HRID 2261879Notes

Reaction equation

HRID 2261879 reaction equation
[P:1]([O:13]C[C@H]1O[C@@H](N2C=CC(N)=NC2=O)C[C@@H]1O)([O:4][P:5]([O:8][P:9]([OH:12])([OH:11])=[O:10])([OH:7])=[O:6])(=[O:3])[OH:2].P(O[CH2:42][C@H:43]1[O:47][C@@H:46]([N:48]2[C:58]3[N:57]=[C:55]([NH2:56])[NH:54][C:52](=O)[C:51]=3[N:50]=[CH:49]2)[CH2:45][C@@H:44]1[OH:59])(OP(OP(O)(O)=O)(O)=O)(=O)O>[Au]>[CH:55]1[N:57]=[C:58]2[N:48]([C@@H:46]3[O:47][C@H:43]([CH2:42][O:12][P:9]([O:8][P:5]([O:4][P:1]([OH:13])([OH:3])=[O:2])([OH:7])=[O:6])([OH:11])=[O:10])[C@@H:44]([OH:59])[CH2:45]3)[CH:49]=[N:50][C:51]2=[C:52]([NH2:54])[N:56]=1

Participants

4

Reactant 2

HCID 65091dCTPHCID 135398599dGTP

Product 1

HCID 15993Datp

Reagents, catalysts & solvents 1

HCID 23985GoldCatalyst

Procedure

The reaction conditions for the PCR followed a protocol recommended by the enzyme manufacturer (PE Applied Biosystems, Foster City, Calif.). The Banyuls promoter was isolated from Arabidopsis genomic DNA using 30 nanomoles each of primers Ban+1500 and Ban GSP1, 10 micromoles each of dATP, dCTP, dGTP, and TTP, 2.5 units of AmpliTaq Gold in 1× Opti-Prime™ Buffer 3 (Stratagene, La Jolla, Calif.). After an initial incubation at 95° C. for 10 minutes, 30 cycles of PCR were performed with 92° C. for 30 seconds, 56° C. for 30 seconds, and 72° C. for 2 minutes followed by 1 cycle of 72° C. for 7 minutes.

Workup

  1. customThe reaction conditions for the PCR

Sources & evidence

ORD record
ord-a5f2b4abb6fa4deeaf9edda2c0f9aa4e
Source dataset
uspto-grants-2010_12
Patent number
US07847153B2

Reaction notes

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