反应详情

Fmoc-Arg(OtBu)-OH, 1-mesitylenesulfonyl-3-nitro-1,2,4-triazole (MSNT) and N-methylimidazole were dissolved in CH2Cl2, and the solution was stirred for 5 minutes and thereafter placed into the solid-phase synthesis column containing the resin, followed by stirring at room temperature for 3 hours. The resin was thereafter washed with methylene chloride, isopropanol and DMF and dried. The unreacted hydroxyl group on the solid phase was thereafter acetylated using a 20% DMF solution of acetic anhydride for 20 minutes for capping. The resin was washed with DMF and stirred along with a 20% piperidine/DMF solution for 20 minutes to remove the protective Fmoc group, whereby resin-Arg-NH2 was obtained. The product was washed with DMF and dried.

1. customplaced into the solid-phase synthesis column
2. additioncontaining the resin
3. stirringby stirring at room temperature for 3 hours
4. washThe resin was thereafter washed with methylene chloride, isopropanol and DMF
5. customdried
6. customfor 20 minutes
7. washThe resin was washed with DMF
8. stirringstirred along with a 20% piperidine/DMF solution for 20 minutes
9. customto remove the protective Fmoc group