Phosphate → Acridine Orange Ethidium Bromide

Beteiligte Stoffe

Reaktionsschritte

Apoptosis was determined morphologically after staining with acridine orange and ethidium bromide and counting viable cells by fluorescence microscopy. Cell density was adjusted to 1×106 cells/ml in buffer. Acridine orange and ethidium bromide (1:1) (v/v) mixed in PBS (phosphate buffer solution) to a final concentration of 100 μg/ml. 2 μl dye was mixed with 25 μl of cell suspension and cells were immediately checked.

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