反应详情

温度

65 °C

To a stirred solution of 1-cyclopropyl-3-(2-fluoro-4-iodo-phenylamino)-1H-pyrrolo[3,2-c]pyridine-2-carboxylic acid methyl ester (150.0 mg, 0.33 mmol) in anhydrous THF (3.5 mL) and anhydrous methanol (1.0 mL) under N2 was added freshly prepared 1N aqueous sodium hydroxide (0.66 ml, 2.0 eq). The reaction mixture was stirred at 65° C. for 1 h. The resultant reaction mixture was evaporated in vacuo and azeotroped with toluene (3×5 ml) to give a yellow solid. The yellow solid was redissolved in anhydrous THF (6.6 ml). EDCI (127.0 mg, 0.66 mmol, 2.0 eq) and HOBT (89.7 mg, 0.66 mmol, 2.0 eq) were added. The reaction mixture was stirred at room temperature. After 5 minutes O-(2-vinyloxy-ethyl)-hydroxylamine (68.5 mg, 0.66 mmol, 2.0 eq) and DIPEA (0.12 mL, 0.66 mmol, 2.0 eq) were added, and the reaction mixture was stirred at ambient temperature under N2 for 16 h. The reaction mixture was absorbed onto silica and purified by flash column chromatography (silica, ISCO, 45 mL/min, 0 to 20% EtOH in DCM) to give the desired product as a pale yellow solid (57.5 mg, 33.1%). 1H-NMR (400 MHz, CD3OD) δ ppm 8.72 (s, 1H), 8.24 (d, J=6.0 Hz, 1H), 7.54-7.32 (m, 2H), 7.21 (ddd, J=8.5, 1.1 Hz, 1H), 6.61-6.39 (m, 1H), 3.86-3.70 (m, 2H), 3.62 (t, J=6.0 Hz, 2H), 3.35-3.24 (m, 1H), 3.34-3.32 (m, 1H), 3.14-3.00 (m, 2H), 1.42-1.27 (m, 2H), 0.94-0.78 (m, 2H); LC-MS (method D): [M+H]+=523.2, RT=2.21 min.

1. customThe resultant reaction mixture
2. customwas evaporated in vacuo
3. customazeotroped with toluene (3×5 ml)
4. customto give a yellow solid
5. stirringThe reaction mixture was stirred at room temperature
6. stirringthe reaction mixture was stirred at ambient temperature under N2 for 16 h
7. customThe reaction mixture was absorbed onto silica
8. custompurified by flash column chromatography (silica, ISCO, 45 mL/min, 0 to 20% EtOH in DCM)