反应详情

Crude 6-amino-9-benzyl-8-hydroxy-2-mercaptopurine (150 mg, 0.55 mmol) was suspended in dimethylformamide (10 ml). To the suspension were added potassium carbonate (202 mg, 1.46 mmol) and 4-chlorobutyronitrile (152 mg, 1.46 mmol) in order. The mixture was stirred at room temperature for 3 hours. The solvent was removed in vacuo, and to the residue was added water. The mixture was extracted with chloroform and the organic layer was dried on sodium sulfate. After removal of the solvent in vacuo, the residue was purified by silica gel chromatography (5% methanol/chloroform) to give the subject compound (71 mg, yield 29%).

1. customThe solvent was removed in vacuo
2. additionto the residue was added water
3. extractionThe mixture was extracted with chloroform
4. customthe organic layer was dried on sodium sulfate
5. customAfter removal of the solvent in vacuo
6. customthe residue was purified by silica gel chromatography (5% methanol/chloroform)