反应详情

温度

20 °C

In a three-necked flask under argon, 0.5 g of the sodium salt of [4-(2-fluoromethylmorpholin-4-yl)-6-oxo-1,6-dihydropyrimidin-2-yl]acetic acid previously obtained (example 30b, step 3b) is placed in 7 ml of pyridine and 7 ml of dimethylformamide, and then N-[3-(dimethylamino)propyl]-N′-ethylcarbodiimide hydrochloride is added at ambient temperature, followed by 0.25 g of (S)-2-methylindoline (CAS 22160-09-4). The reaction medium is stirred at ambient temperature (20° C.) for 72 hours. 25 ml of water are added, extraction is carried out with 3 times approximately 25 ml of dichloromethane, washing is carried out with 25 ml of water, washing is carried out with twice approximately 15 ml of 1M hydrochloric acid solution, washing is carried out with 25 ml of water, washing is carried out with 25 ml of saturated NaCl solution, drying is carried out over MgSO4, filtration is carried out through a VF filter, and the resulting product is concentrated under vacuum. The compound obtained is chromatographed on silica gel (40-63 μm), elution being carried out with a mixture of dichloromethane and ethanol (95/5, v/v). The fractions containing the expected compound are combined and evaporated. The compound obtained is triturated from 1 ml of dichloromethane and 10 ml of diisopropyl ether, filtered, and dried at ambient temperature (20° C.). (+)-6-(2-Fluoromethylmorpholin-4-yl)-2-[2-((S)-2-methyl-2,3-dihydroindol-1-yl)-2-oxoethyl]-3H-pyrimidin-4-one is isolated (0.315 g).

1. extractionextraction
2. washwashing
3. washwashing
4. washwashing
5. washwashing
6. customdrying
7. filtrationis carried out over MgSO4, filtration
8. filtrationfilter
9. concentrationthe resulting product is concentrated under vacuum
10. customThe compound obtained
11. customis chromatographed on silica gel (40-63 μm), elution
12. additionbeing carried out with a mixture of dichloromethane and ethanol (95/5
13. additionThe fractions containing the expected compound
14. customevaporated
15. customThe compound obtained
16. customis triturated from 1 ml of dichloromethane and 10 ml of diisopropyl ether
17. filtrationfiltered
18. customdried at ambient temperature (20° C.)