Diethylethanolamine → Indoxyl acetate

HRID 2285762Notes

Reaction equation

HRID 2285762 reaction equation
S(CCCN1CCN(CCCS(O)(=O)=O)CC1)(O)(=O)=[O:2].CCN(CCO)CC.CC[CH:31]([CH2:34][O:35][C:36]([C:49]([N:51](CC[NH+](C)C)C)=O)(C1C=CC=CC=1)[C:37]1[CH:42]=[CH:41][CH:40]=[CH:39][CH:38]=1)CC.[Cl-]>>[CH3:31][C:34]([O:35][C:36]1[C:37]2[C:42](=[CH:41][CH:40]=[CH:39][CH:38]=2)[NH:51][CH:49]=1)=[O:2]

Participants

4

Reactant 3

HCID 7497DiethylethanolamineHCID 19153X 100HCID 797241,4-Piperazinedipropanesulfonic acid

Product 1

HCID 11841Indoxyl acetate

Procedure

In accordance with the procedures described previously, 10 mg of eel cholinesterase (Code ECH, Worthington Biochemical Company, approximately 100 units/mg) is dissolved in 30 ml of the 0.5 M, pH 8.0, "TRITON X-100"-containing 1,4-bis(3-sulfopropyl) piperazine buffer solution described previously. Then 30 microliters of the resulting buffered eel cholinesterase solution (i.e. 1.0 unit of the enzyme) were impregnated into 12.7 mm discs of DE81 (diethylaminoethyl ion exchange paper, 3.5 microequivalents per cm2) paper (DEAE) discs, allowed to air-dry for 3 hours at room temperature, placed in uncapped glass vials and allowed to dry for 12 hours at 0.01 mm Hg, at 25° C. in a vacuum desiccator. Indoxyl acetate discs were prepared from the following solution and 9.0 cm Whatman No. 1 filter paper circles.

Workup

  1. customto air-dry for 3 hours at room temperature
  2. customto dry for 12 hours at 0.01 mm Hg, at 25° C. in a vacuum desiccator
  3. filtration1 filter paper circles

Sources & evidence

ORD record
ord-8de5eec15523491b9418ff7a130de464
Source dataset
uspto-grants-1982_04
Patent number
US04324858

Reaction notes

Loading…