Phenol → 2-Hydroxymuconic semialdehyde

HRID 2294768Notes

Reaction equation

C1([OH:7])C=CC=CC=1.CC1(C)S[C@@H]2[C@H](NC([C@H](N)C3C=CC=CC=3)=O)C(=O)N2[C@H]1C(O)=O.C[C@:33]1([OH:63])[C@@H:47]2[C:42](=[C:43](O)[C@:44]3([OH:60])[C:51](=[O:52])C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]3C2)C(=O)C2C(O)=CC=CC1=2>>[CH:42](/[CH:47]=[CH:33]/[OH:63])=[CH:43]\[C:44]([C:51]([OH:7])=[O:52])=[O:60]

Participants

Reactant 3

HCID 996Phenol

HCID 6249Ampicillin

HCID 178418840HCID 178418840, compound

Product 1

HCID 52803662-Hydroxymuconic semialdehyde

Procedure

Transformants were spread on LB plates containing 1 mM phenol, 50 μg of ampicillin per ml, and 12.5 μg of tetracycline per ml and incubated overnight at 37° C. Colonies that became yellow (as a result of 2-hydroxymuconic semialdehyde) following spraying of a 0.1% of catechol solution were patched on LB plates containing 1 mM phenol. Colonies in which the yellow compound formed during growth without catechol addition were retained as putative carriers of both pheA and pheB.

Sources & evidence

ORD record: ord-3bb1dad6fd25457ea2ea838c8652e91e
Source dataset: uspto-grants-1997_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05691171

Reaction notes

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