Ethylene Glycol → (2R,3S,4S,5R)-2,3,4,5,6-pentahydroxyhexanal

Participants

Reaction Steps

Periodate oxidations. The polysaccharide (0.3 mM galactose equivalents) was dissolved in phosphate buffer (20 ml) as before and 1-propanol (1 ml) was added followed by aqueous solutions (2 ml) of sodium metaperiodate (0.23 mM/mol hexose unit for guaran and 0.17 mM/mol hexose unit for locust bean gum). The oxidation was conducted at 5° C. in the dark for 15 hr after which it was stopped by addition of ethylene glycol (1 ml). When the oxidation was carried out in smaller solution volumes (~8 ml) the same ropy gels were obtained as in the case of galactose oxidase treatments. The dialdehyde products were isolated after dialysis (3 d).

Provenance & Evidence