Hydrochloric Acid → HCID 131713365, compound

Participants

Reaction Steps

Conc. hydrochloric acid (1.5 g) was added to the mixture of 7-[2-methylthiomethoxyimino-2-(2-formamido-5-chlorothiazol-4-yl)acetamido]-3-(1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (2.8 g) and methanol (30 ml), and stirred at ambient temperature for 5 hours. After removal of solvent, and the residue was dissolved in water and the solution was adjusted to pH 4.0 with 10% aqueous sodium hydroxide under ice-cooling. The solution was subjected to column chromatography on macroporus non-ionic adsorption resin "Diaion HP-20" and eluted with 20% aqueous solution of isopropyl alcohol. The fractions containing the object compound were collected and concentrated and lyophilized to give 7-[2-methylthiomethoxyimino-2-(2-amino-5-chlorothiazol-4-yl)acetamido]-3-(1-pyridiniomethyl)-3-cephem-4-carboxylate (syn isomer) (1.7 g).

Provenance & Evidence