Citric Acid → HCID 9916797, compound

Participants

Reaction Steps

(2S)-3-(4-Biphenylyl)-N-butyl-2-[3-[(1RS)-1-(ethoxycarbonylmethyl)-2-(phenylthio)ethyl]-3-isoamylureido]propionamide (Compound No. 38-1, 402 mg) is dissolved in ethanol(2 ml), a 1 N aqueous lithium hydroxide solution (0.96 ml) is added thereto, and the mixture is stirred at room temperature for 45minutes. A 10% aqueous citric acid solution is added to the reaction mixture, and the whole is extracted with ether. The organic layer is washed with water and saturated brine successively, dried over anhydrous magnesium sulfate and concentrated under reduced pressure. The resulting oily matter is purified by silica gel column chromatography to give 386 mg (quantitatively) of the titled compound (Compound No. 39-1) as noncrystalline powder.

Provenance & Evidence