Chloramphenicol → HCID 178515956, compound

HRID 2302136Notes

Reaction equation

HRID 2302136 reaction equation
C[C@H]1O[C@H]2O[C@H:10]3[C@H:15](O[C@@]2(O)C(=O)C1)[C@@H:14](NC)[C@@H:13](O)[C@@H:12]([NH:20]C)[C@@H:11]3[OH:22].CC1(C)S[C@@H]2[C@H](NC([C@H](N)C3C=CC=CC=3)=O)C(=[O:32])N2[C@H]1C(O)=O.[CH:48]1[C:53]([C@@H](O)[C@H](NC(C(Cl)Cl)=O)CO)=[CH:52][CH:51]=[C:50]([N+:65]([O-])=O)[CH:49]=1.C(O)CCO>>[CH:52]1[CH:51]=[C:50]([NH:65]/[N:20]=[C:12]2\[CH:13]=[CH:14][CH:15]=[CH:10][C:11]\2=[O:22])[C:49]([OH:32])=[CH:48][CH:53]=1

Participants

5

Reactant 4

HCID 5959ChloramphenicolHCID 6249AmpicillinHCID 104421,3-PropanediolHCID 15541Spectinomycin

Product 1

HCID 178515956HCID 178515956, compound

Procedure

coli strain FM5 was transformed with the dha plasmid pDT24 (specR), the btuB plasmids pBtuB1 (ampR) or pBtuB2 (chlR), or the btuBCED plasmid pBCED (chlR). Selection is on LB plates containing 50 mg/L spectinomycin, 50 mg/L ampicillin or 100 mg/L chloramphenicol. Colonies resistant to the appropriate antibiotics were used for 1,3-propanediol production and vitamin or coenzyme B12 uptake.

Sources & evidence

ORD record
ord-1cebfe767628491880a243f7b1e81576
Source dataset
uspto-grants-2002_08
Patent number
US06432686B1

Reaction notes

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