HRID2302136

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：O=C(N[C@H](CO)[C@H](O)c1ccc([N+](=O)[O-])cc1)C(Cl)Cl

HCID5959

Chloramphenicol

C11H12Cl2N2O5

结构：CC1(C)S[C@@H]2[C@H](NC(=O)[C@H](N)c3ccccc3)C(=O)N2[C@H]1C(=O)O

HCID6249

Ampicillin

C16H19N3O4S

HCID10442

1,3-Propanediol

C3H8O2

结构：CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@@]3(O)C(=O)C[C@@H](C)O[C@H]3O[C@@H]2[C@H]1O

HCID15541

Spectinomycin

C14H24N2O7

PRODUCTS

生成物

$结构：O=C1C=CC=C/C1=N\Nc1ccccc1O$

HCID178515956

未命名化合物

PROCEDURE

实验过程

coli strain FM5 was transformed with the dha plasmid pDT24 (specR), the btuB plasmids pBtuB1 (ampR) or pBtuB2 (chlR), or the btuBCED plasmid pBCED (chlR). Selection is on LB plates containing 50 mg/L spectinomycin, 50 mg/L ampicillin or 100 mg/L chloramphenicol. Colonies resistant to the appropriate antibiotics were used for 1,3-propanediol production and vitamin or coenzyme B12 uptake.