Pseudoephedrine → (-)-Pseudoephedrine

Participants

Reaction Conditions

Reaction Steps

Coprinus rhizophorus IFO 30197 was inoculated to a medium containing 1% glucose, 0.5% peptone and 0.3% yeast extract, and culturing was aerobically carried out at 30° C. for 48 hours. After this cultured solution (5 ml) was centrifuged to give bacterial cells, the cells were dried in the air and the resulting dried bacterial cells were suspended in 1 ml of 0.05 M Tris hydrochloric acid buffer (pH 7.5). To this were added glucose (50 mg), glucose dehydrogenase (0.2 mg), NADP (0.6 mg), NAD (0.6 mg) and dl-2-methylamino-1-phenyl-1-propanone hydrochloride (10 mg). Reaction was allowed to take place by carrying out reciprocation-shaking at 28° C. and at 300 rpm for 48 hours. The reaction solution was analyzed on HPLC (Column Sumichiral AGP manufactured by Sumika Chemical Analysis Service; diameter of 4 mm; length of 150 mm; 0.03 M sodium phosphate buffer; pH 7.0; flow rate of 0.5 ml/min; detection wavelength at UV 220 nm). The produced amounts and the optical activities of pseudoephedrine were determined. Consequently, only l-pseudoephedrine was obtained selectively, as shown in Table 6.

Provenance & Evidence