Magnesium Sulfate Heptahydrate → L-Aspartic Acid

Participants

Reaction Conditions

Reaction Steps

Escherichia coli TA 5004 was inoculated into a medium (500 ml, pH 7.0) containing ammonium fumarate (3%), potassium dihydrogen phosphate (0.2%), magnesium sulfate heptahydrate (0.05%), corn steep liquor (4%) and yeast extract (2%) and incubated with shaking at 37° C. for 24 hours. The resulting culture was adjusted to pH 8.5 with aqueous ammonia. Ammonium fumarate (65 g) and Triton X-100 (500 mg) were added to the culture and incubation was further continued at 37° C. for 6 hours to conduct the enzymatic reaction. The reaction mixture was filtered, concentrated and adjusted to pH 2.8 to precipitate crude crystals of L-aspartic acid. The crude crystals were filtered off and recrystallized from water to give L-aspartic acid (47.0 g).

Provenance & Evidence