Glutaraldehyde → L-Aspartic Acid

HRID 2308930Notes

Reaction equation

HRID 2308930 reaction equation
C(=O)CCCC=O.[C:8]([O-:15])(=[O:14])/[CH:9]=[CH:10]/[C:11]([O-:13])=[O:12].[NH4+:16].[NH4+].[Cl-].[Mg+2].[Cl-]>P([O-])([O-])([O-])=O>[NH2:16][C@H:9]([C:8]([OH:15])=[O:14])[CH2:10][C:11]([OH:13])=[O:12]

Participants

5

Reactant 3

HCID 3485GlutaraldehydeHCID 5360315Magnesium ChlorideHCID 9793847Ammonium fumarate

Product 1

HCID 5960L-Aspartic Acid

Reagents, catalysts & solvents 1

HCID 1061PhosphateSolvent2×

Procedure

The microbial cells of Escherichia coli TA5004 (10 g) was suspended in 0.05 M phosphate buffer (pH 8.5, 50 ml) and sonicated at 9 Kc for 15 minutes. Then, the suspension was centrifuged and the resulting supernatant (40 ml) was passed through a column packed with Duolite-A7 (a weak basic anion exchange resin manufactured by Diamond Shamrock Chemical Co. in U.S.A.) (60 ml) at room temperature at 30 ml/hr. Then, the column was subjected to cross-linking treatment with a solution containing 0.1 M phosphate buffer (pH 8.5, 300 ml) and 0.4% glutaraldehyde (300 ml) for 30 minutes and excess glutaraldehyde was thoroughly washed out to give an immobilized enzyme. The immobilized enzyme was packed in a column (volume 50 ml) and 1 M ammonium fumarate solution containing 1 mM magnesium chloride (pH 8.5, 500 ml) was passed through the column at the flow rate of 25 ml/hr. The combined effluent was adjusted to pH 2.8 to precipitate crystals. The crystals were filtered off to give L-aspartic acid (55 g).

Workup

  1. customsonicated at 9 Kc for 15 minutes
  2. customat room temperature
  3. washexcess glutaraldehyde was thoroughly washed out
  4. customto give an immobilized enzyme
  5. customto precipitate crystals
  6. filtrationThe crystals were filtered off

Sources & evidence

ORD record
ord-5fe3dd5c56f84bd8b5e6de1065a028fc
Source dataset
uspto-grants-1987_09
Patent number
US04692409

Reaction notes

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