Dimethylformamide → Z-D-Lys(Boc)-Phe-Gly

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Reaction Steps

1.26 g of G-Gly-OMe.HCl, 1.6 g of HOObt, 1.3 ml of NEM and 2.2 g of DCC are added in succession, with stirring, to 5.3 g of Z-D-Lys(Boc)-Phe-OH (Example 1d) in 60 ml of DMF. After the mixture has been left to stand overnight, the urea is filtered off and the filtrate is concentrated in vacuo. The residue is taken up in 200 ml of ethyl acetate and the solution is washed in the cold with 10% strength citric acid solution, saturated sodium bicarbonate solution and water and concentrated in vacuo to about 40 ml, and ether/petroleum ether 1:1 are added to the residue. The precipitate is filtered off, washed with ether/petroleum ether 1:1 and dried.

Provenance & Evidence