Phosphate → Phosphate Buffered Saline

HRID 232508Notes

Reaction equation

[P:1]([O-:5])([OH:4])([OH:3])=[O:2].[K+:6].[P:7]([O-:11])([OH:10])([OH:9])=[O:8].[Na+:12].[Cl-:13].[Na+].P([O-])([O-])([O-])=O.[N-]=[N+]=[N-].[Na+]>>[OH:3][P:1]([O-:5])([OH:4])=[O:2].[OH:9][P:7]([O-:11])([O-:10])=[O:8].[Na+:12].[Na+:12].[Na+:12].[Cl-:13].[Cl-:13].[K+:6].[K+:6]

Participants

Reactant 5

HCID 1061Phosphate

HCID 5234Sodium Chloride2×

HCID 33557Sodium Azide

HCID 516951Monobasic potassium phosphate

HCID 23672064Monosodium phosphate

Product 1

HCID 24978514Phosphate Buffered Saline

Procedure

A buffer was prepared from potassium dihydrogen phosphate, sodium dihydrogen phosphate (12 hydrate) and sodium chloride to make phosphate concentration of 0.036M, NaCl concentration of 0.156M and pH 6.5. To this buffer was added NaN3 at a concentration of 0.1% (W/V).

Sources & evidence

ORD record: ord-ca21acdb96284994ba43524996abab8f
Source dataset: uspto-grants-1997_01
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US05591596

Reaction notes

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