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HRID234590

反应详情

EQUATION

反应方程式

HRID 234590 的结构方程式

REACTANTS

反应物

6
结构：CC(=O)[O-]
HCID175

Acetate

C2H3O2-

结构：O=P([O-])([O-])[O-]
HCID1061

Phosphate

O4P-3

结构：[O-]B([O-])[O-]
HCID26574

Borate

BO3-3

结构：O=C([O-])O.O=C([O-])[O-]
HCID86586141

未命名化合物

结构：NCC(=O)O.[Na+].[OH-]
HCID86654294

未命名化合物

结构：CC(=O)[O-].CCC1(CC)C(=O)NC(=O)NC1=O.Cl.[Na+]
HCID131719622

未命名化合物

PRODUCTS

生成物

1
结构：O=C(O)CN(CCN(CC(=O)O)CC(=O)O)CC(=O)O
HCID6049

Ethylenediaminetetraacetic acid

C10H16N2O8

PROCEDURE

实验过程

Any buffer providing the desired pH which does not interfere the measurement of enzyme activity is suitable for use in the present invention. However, more specifically, a conventional acetate buffer can be used to provide a pH of 6.0 or less; a conventional phosphate buffer can be used to provide a pH of from 5.5 to 9.5; a carbonate-bicarbonate buffer (e.g., K2CO3 --NaHCO3) buffer can be used to provide a pH of from 8.5 to 11.0; a conventional Britton-Robinson buffer can be used to provide a pH of from 5.0 to 9.0; a conventional Veronal-sodium acetate-HCl buffer can be used to provide a pH of from 3.0 to 9.0; a TRIS-HCl buffer can be used to provide a pH of from 6.8 to 8.5; a conventional glycine-NaOH buffer can be used to provide a pH of from 8.5 to 9.5; a conventional borate buffer can be used to provide a pH of from 8.0 to 11.0; a conventional EDTA buffer can provide a pH of about 8.5; etc.

WORKUP

后处理

  1. customAny buffer providing the desired pH which
  2. customto provide a pH of 6.0 or less
  3. customto provide a pH of from 5.5 to 9.5
  4. customto provide a pH of from 8.5 to 11.0
  5. customto provide a pH of from 5.0 to 9.0
  6. customto provide a pH of from 3.0 to 9.0
  7. customto provide a pH of from 6.8 to 8.5
  8. customto provide a pH of from 8.5 to 9.5
  9. customto provide a pH of from 8.0 to 11.0