Ethylenediaminetetraacetic Acid → HCID 86674305, compound

HRID 2358920Notes

Reaction equation

HRID 2358920 reaction equation
[CH2:1]([N:12]([CH2:17][C:18]([OH:20])=[O:19])[CH2:13][C:14]([OH:16])=[O:15])[CH2:2][N:3]([CH2:8][C:9]([OH:11])=[O:10])[CH2:4][C:5]([OH:7])=[O:6].[C:21]([O-:24])(=[O:23])[CH3:22].[Na+:25]>C(O)C>[CH2:5]([OH:6])[CH3:4].[CH2:2]([N:3]([CH2:8][C:9]([OH:11])=[O:10])[CH2:4][C:5]([OH:7])=[O:6])[CH2:1][N:12]([CH2:17][C:18]([OH:20])=[O:19])[CH2:13][C:14]([OH:16])=[O:15].[C:21]([O-:24])(=[O:23])[CH3:22].[Na+:25]

Participants

4

Reactant 2

HCID 6049Ethylenediaminetetraacetic AcidHCID 517045Sodium Acetate

Product 1

HCID 86674305HCID 86674305, compound

Reagents, catalysts & solvents 1

HCID 702EthanolSolvent

Procedure

2 μL of 125 mM EDTA and 2 μL of 3 M sodium acetate are added to each well. And then 50 μL 100% ethanol is added to each well. The plate is sealed and mixed by inverting 4 times. The plate is incubated at room temperature for 15 minutes. Then the precipitated DNA is washed with 75% ethanol for 3 times.

Workup

  1. customThe plate is sealed
  2. additionmixed
  3. washThen the precipitated DNA is washed with 75% ethanol for 3 times

Sources & evidence

ORD record
ord-73a2724085a0476c89499840040c926d
Source dataset
uspto-grants-2014_04
Patent number
US08685647B2

Reaction notes

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