Diisopropylcarbodiimide → (S)-2,5-dioxopyrrolidin-1-yl 2-(methoxycarbonylamino)-3-methylbutanoate

Temperature

2.5 °C

Compound I can be prepared according to the procedures described in U.S. Provisional Patent Application No. 61/243,596, filed Sep. 18, 2009. To a mixture of (S)-2-(methoxycarbonylamino)-3-methylbutanoic acid (19.66 g, 112 mmol) and N-hydroxysuccinimide (13.29 g, 116 mmol) was added ethyl acetate (250 ml), and the mixture was cooled to 0-5° C. Diisopropylcarbodiimide (13.88 g, 110 mmol) was added and the reaction mixture was stirred at 0-5° C. for about 1 hour. The reaction mixture was warmed to room temperature. The solids (diisopropylurea by-product) were filtered and rinsed with ethyl acetate. The filtrate was concentrated in vacuo to an oil. Isopropyl alcohol (200 ml) was added to the oil and the mixture was heated to about 50° C. to obtain a homogeneous solution. Upon cooling, crystalline solids formed. The solids were filtered and washed with isopropyl alcohol (3×20 ml) and dried to give (S)-2,5-dioxopyrrolidin-1-yl 2-(methoxycarbonylamino)-3-methylbutanoate as a white solid (23.2 g, 77% yield).

1. temperatureThe reaction mixture was warmed to room temperature
2. filtrationThe solids (diisopropylurea by-product) were filtered
3. washrinsed with ethyl acetate
4. concentrationThe filtrate was concentrated in vacuo to an oil
5. additionIsopropyl alcohol (200 ml) was added to the oil
6. temperaturethe mixture was heated to about 50° C.
7. customto obtain a homogeneous solution
8. temperatureUpon cooling
9. customcrystalline solids formed
10. filtrationThe solids were filtered
11. washwashed with isopropyl alcohol (3×20 ml)
12. customdried

ORD record

ord-fa6dd6336d624c3ca893062f5845b40d

Source dataset

uspto-grants-2014_04

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08686026B2