Sodium Hydroxide → HCID 15595920, compound

Participants

Reaction Steps

A 3.2 ml quantity of a 1N aqueous solution of sodium hydroxide was added with ice cooling and stirring to a solution of 600 mg of the N-[(R)-1-ethoxycarbonyl-2-hexylthioethyl]-alanyl-(S)-proline (α-isomer) obtained in Example 4 in 8 ml of ethanol. The mixture was stirred at room temperature for 2.5 hours, and applied to a column (Dowex 50W-X8(H+)). The reaction product was sufficiently washed with water, and eluted with a 4% aqueous solution of pyridine. Fractions of the title compound were collected and lyophilized. The powder thus obtained was reprecipitated with ethanolether, giving the title compound. Yield 270 mg.

Provenance & Evidence