Sodium Hydroxide → HCID 13647973, compound

Participants

Reaction Steps

A 2.0 ml quantity of a 1N aqueus solution of sodium hydroxide was added with ice cooling and stirring to a solution of 373 mg of the N-[(R)-1-ethoxycarbonyl-2-heptylthioethyl]-alanyl-(S)-proline (β-isomer) prepared in Example 17 in 4 ml of ethanol. The mixture was stirred at room temperature for 3 hours and applied to a column (Dowex 50W-X8(H+)). The reaction product was sufficiently washed with water and was eluted with a 4% aqueous solution of pyridine. Fractions of the title compound were collected and lyophilized. The powdery substance thus obtained was reprecipitated with ethanol-ether, giving the title compound.

Provenance & Evidence