Trifluoroacetic acid → HCID 58493941, compound

Participants

Reaction Steps

220 mg tert-butyl (S)-3-{4-[2-(5-tert-butyl-3-methanesulphonylamino-2-methoxy-phenylcarbamoyl)-1-methyl-1H-indol-7-ylmethyl]-piperazine-1-carbonyl}-pyrrolidine-1-carboxylate are dissolved in 5 ml dichloromethane, combined with 231 μl trifluoroacetic acid and stirred for 12 hours at ambient temperature. Then the solvents are eliminated in vacuo, divided between saturated aqueous sodium hydrogen carbonate solution and dichloromethane and stirred vigorously for 30 minutes. The phases are separated, the aqueous phase is extracted twice with dichloromethane and the combined organic phases are dried on magnesium sulphate. Then the solvents are eliminated in vacuo and the crude product is chromatographed on silica gel (dichloromethane/(methanol-conc. ammonia 9:1) 90:10 to 80:20).

Provenance & Evidence