L-Proline → L-Alanyl-L-proline

Participants

Reaction Conditions

Reaction Steps

L-Ala-OMe.HCl was added to the L-Pro-TBP prepared by the conversion of L-Pro into its ionic liquid form according to the method disclosed in Example 1 so as to control the concentration of the L-Ala-OMe.HCl to 1.000 mmol per unit mass (1 kg) of the L-Pro-TBP (the amount of water relative to the total mass of the reaction system: 2% by mass). The mixture was stirred enough to ensure the mixture uniform, and then, the reaction of these components was initiated while stirring the reaction system in a water bath and warming the same at 37° C. or 50° C. The reaction was finished 72 hours after the initiation of the reaction, and the samples, which were collected at appropriate intervals of time during the reaction, were analyzed according to the HPLC technique to confirm the formation of the desired dipeptide, i.e., L-Ala-Pro. The results are summarized in the following Table 1.

Provenance & Evidence