Hydrochloric Acid → HCID 67969363, compound

Participants

Reaction Steps

tert-Butyl 7-(4-(4-fluorophenethyl)-2-oxopiperazin-1-yl)-1,1,5-trimethyl-3,4-dihydro-1H-pyrido[4,3-b]indole-2(5H)-carboxylate (106 mg, 0.240 mmol) was dissolved in MeOH (5.00 mL), and 2 M HCl in Et2O (1.20 mL) was added. The reaction was allowed to proceed for 18 h. The mixture was concentrated, and the residue was partitioned between CH2Cl2 and saturated NaHCO3 solution. The organic phase was separated, and the aqueous phase was back extracted with CH2Cl2. The combined organic extracts were dried over Na2SO4, filtered and concentrated to dryness. Purification by preparative thin layer chromatography (silica gel, 90:9:1 CH2Cl2/MeOH/NH4OH) gave the free base of the title compound (19.0 mg) as a white solid. The free base was dissolved in MeOH (2.00 mL) and treated with 1.25 M HCl in MeOH (0.108 mL). The mixture was concentrated to provide the title compound (22.0 mg, 17%) as an off-white solid. Free Base: 1H NMR (500 MHz, CDCl3) δ 7.57 (d, J=8.5 Hz, 1H), 7.21-7.17 (m, 3H), 7.02-6.94 (m, 3H), 3.75 (t, J=5.0 Hz, 2H), 3.59 (s, 3H), 3.43 (s, 2H), 3.26 (t, J=5.5 Hz, 2H), 2.90 (t, J=5.5 Hz, 2H), 2.86-2.82 (m, 2H), 2.74-2.69 (m, 4H), 1.55 (s, 6H); HPLC (Method B) 97.0% (AUC), tR=10.7 min. HCl salt: 1H NMR (500 MHz, CD3OD) δ 7.69 (d, J=8.5 Hz, 1H), 7.45 (s, 1H), 7.39-7.35 (m, 2H), 7.12-7.06 (m, 3H), 4.20-4.04 (m, 4H), 3.71-3.65 (m, 7H), 3.45-3.44 (m, 2H), 3.17-3.14 (m, 4H), 1.85 (s, 6H); ESI MS m/z 435 [M+H]+; HPLC (Method B) 95.2% (AUC), tR=10.8 min.

Provenance & Evidence