Sodium Hydroxide → 4-{[6-Cyclopropyl-3-(pyrimidin-5-ylamino)-pyridine-2-carbonyl]-amino}-1-methyl-1H-pyrazole-3-carboxylic acid

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Reaction Steps

To a stirred solution of 4-{[6-cyclopropyl-3-(pyrimidin-5-ylamino)-pyridine-2-carbonyl]-amino}-1-methyl-1H-pyrazole-3-carboxylic acid methyl ester (example 27, step 4; 300 mg, 0.76 mmol) in MeOH (7 ml) was added a solution of NaOH (49 mg, 1.2 mmol) in water (2 ml) while keeping the internal bath temperature below 5° C. The reaction mixture was stirred for 12 h at rt, then concentrated. The residue was dissolved in water (10 ml), and the aqueous layer was washed with EtOAc. The aqueous phase was acidified with aqueous 1N HCl solution (pH˜4-5), then saturated with sodium chloride, and extracted with EtOAc. Combined organic layers were washed with brine, dried (Na2SO4), and filtered. The organic layer was concentrated to give the title compound (142 mg, 45%) as yellowish sticky solid, which was directly used for next step.

Provenance & Evidence