HCID 605502, compound → HCID 15612355, compound

Participants

Reaction Steps

One gram of 2,4-bismethoxycarbonylbicyclo[3.3.0]octane-3,7-dione is dissolved in 20 ml of methanol and combined with a solution of 2.5 g of α-chymotrypsin in 150 ml of 0.1-molar phosphate buffer pH 7. The solution is extracted with methyl isobutyl ketone after 22 hours of agitation at 28° C.; the extract is concentrated under vacuum, and the remaining residue is chromatographed over a silica gel column (gradient hexane/ethyl acetate), thus obtaining 610 mg of optically pure 2-methoxycarbonylbicyclo[3.3.0]octane-3,7-dione as an oily liquid.

Provenance & Evidence