Leukotriene C4 → Leukotriene C4

Participants

Reaction Steps

Antagonism for LTC4 and LTD4 were determined in isolated guinea-pig trachea prepared as spiral strip. 10 Tracheal preparations were suspended under 1 g tension in ml organ baths and they were incubated for 1 hr prior to use. Contractile responses to LTC4 (2×10-8 g/ml) and LTD4 (2×10-8 g/ml) were obtained after the maximal response to histamine (10-4M). Test compounds dissolved in 100% dimethyl sulfoxide were added to the organ baths (final concentration of 10-5 g/ml) 5 min prior to LTC4 and LTD4 addition, and then contractile responses to LTC4 and LTD4 were compared with those of control which was obtained from a paired trachea in the absence of test compounds. LTC4 - and LTD4 -induced contractions were expressed as a percentage of the maximal response to histamine. The antagonism was determined as follows: Antagonism (%)=(1.0-% contraction in test/% contraction in control)×100

Provenance & Evidence