Hydrochloric Acid → HCID 67896332, compound

Participants

Reaction Steps

A mixture of N-(1-oxo-3-phenylpropyl)-3-[(phenylmethoxy)methyl]-L-histidine, methyl ester (17.2 g., 40.8 mmole) and aqueous sodium hydroxide solution (52 ml. of 1.0N solution, 52 mmole) in methanol (42 ml.) was stirred at 25° for 6 hours, after which it was diluted with water (500 ml.) and washed with ethyl acetate. The aqueous phase was neutralized by the addition of 1.0N hydrochloric acid (52 ml., 52 mmole, resulting pH of 5) and the mixture was extracted with ethyl acetate (5 times). Both the extracts and the aqueous wash were concentrated in vacuo. Each of the concentrates was stirred with hot isopropanol (500 ml. each) and filtered while hot. The filtrates were combined and partially concentrated until crystals formed. The crystals were collected and dried to give 6.5 g. of N-(1-oxo-3-phenylpropyl)-3-[(phenylmethoxy)-methyl]-L-histidine as a white powder; m.p. 163°-165°.

Provenance & Evidence