Hydrochloric Acid → Amphotericin B

Participants

Reaction Steps

In order to study the effect of adding HCl on the stability of amphotericin B, increasing amounts of amphotericin B (i.v. grade) were dissolved in methanol in the presence of one equivalent of HCl (methanolic solution). The degradation in an acid medium of the substance of amphotericin B increases greatly when the amphotericin B concentration is greater than 2 mg/ml (see FIG. 1), even when the addition of HCl in methanolic solution has been carried out at 0° C. (with a 31% contamination). To obtain an amphotericin B/HCl solution at a concentration of 5 mg/ml without degradation of the amphotericin B, amphotericin B was dissolved in methanol in the presence of one equivalent of HCl at a concentration of 2 mg/ml. The solvent was then evaporated off and the salt of amphotericin B with the acid was redissolved in the requisite volume of alcohol.

Provenance & Evidence