1,2-Dioleoyl-sn-glycero-3-phospho(1-rac-glycerol) → Dopg dopc

HRID 2380444Notes

Reaction equation

HRID 2380444 reaction equation
[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH:9]=[CH:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([O:20][CH2:21][CH:22]([O:34][C:35]([CH2:37][CH2:38][CH2:39][CH2:40][CH2:41][CH2:42][CH2:43][CH:44]=[CH:45][CH2:46][CH2:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH3:53])=[O:36])[CH2:23][O:24][P:25]([O:28][CH2:29][CH:30]([OH:33])[CH2:31][OH:32])([OH:27])=[O:26])=[O:19].[CH3:54][CH2:55][CH2:56][CH2:57][CH2:58][CH2:59][CH2:60][CH2:61]/[CH:62]=[CH:63]\[CH2:64][CH2:65][CH2:66][CH2:67][CH2:68][CH2:69][CH2:70][C:71]([O:73][CH2:74][C@@H:75]([O:88][C:89]([CH2:91][CH2:92][CH2:93][CH2:94][CH2:95][CH2:96][CH2:97]/[CH:98]=[CH:99]\[CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH2:106][CH3:107])=[O:90])[CH2:76][O:77][P:78]([O:81][CH2:82][CH2:83][N+:84]([CH3:87])([CH3:86])[CH3:85])([O-:80])=[O:79])=[O:72]>C(Cl)(Cl)Cl>[CH3:1][CH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH:9]=[CH:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][C:18]([O:20][CH2:21][CH:22]([O:34][C:35]([CH2:37][CH2:38][CH2:39][CH2:40][CH2:41][CH2:42][CH2:43][CH:44]=[CH:45][CH2:46][CH2:47][CH2:48][CH2:49][CH2:50][CH2:51][CH2:52][CH3:53])=[O:36])[CH2:23][O:24][P:25]([O:28][CH2:29][CH:30]([OH:33])[CH2:31][OH:32])([OH:27])=[O:26])=[O:19].[CH3:54][CH2:55][CH2:56][CH2:57][CH2:58][CH2:59][CH2:60][CH2:61]/[CH:62]=[CH:63]\[CH2:64][CH2:65][CH2:66][CH2:67][CH2:68][CH2:69][CH2:70][C:71]([O:73][CH2:74][C@@H:75]([O:88][C:89]([CH2:91][CH2:92][CH2:93][CH2:94][CH2:95][CH2:96][CH2:97]/[CH:98]=[CH:99]\[CH2:100][CH2:101][CH2:102][CH2:103][CH2:104][CH2:105][CH2:106][CH3:107])=[O:90])[CH2:76][O:77][P:78]([O:81][CH2:82][CH2:83][N+:84]([CH3:87])([CH3:85])[CH3:86])([O-:80])=[O:79])=[O:72]

Participants

4

Reactant 2

HCID 1191201,2-Dioleoyl-sn-glycero-3-phospho(1-rac-glycerol)HCID 103503171,2-Dioleoyl-sn-glycero-3-phosphocholine

Product 1

HCID 131716291Dopg dopc

Reagents, catalysts & solvents 1

HCID 6212ChloroformSolvent

Procedure

DOPG/DOPC SUV's were prepared by dissolving 40 mg DOPG and 60 mg DOPC in chloroform and drying off the solvent under a stream of nitrogen gas. This dried film was placed under vacuum overnight to remove traces of solvent. The film was then suspended in 2 ml water with vigorous shaking and sonicated in a bath type sonicator for 30 minutes until clear, producing DOPG/DOPC SUV's at a concentration of 50 mg/ml.

Workup

  1. customDOPG/DOPC SUV's were prepared
  2. customdrying off the solvent under a stream of nitrogen gas
  3. customwas placed under vacuum overnight
  4. customto remove traces of solvent
  5. customsonicated in a bath type sonicator for 30 minutes until clear,

Sources & evidence

ORD record
ord-a0f842ff94574774b126e0a68b59cf34
Source dataset
uspto-grants-1994_11
Patent number
US05366737

Reaction notes

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