Leucinamide → N-(tert-Butoxycarbonyl)-L-phenylalanyl-L-leucinamide

Participants

Reaction Steps

1 ml 0.1M veronal-Na/HCl buffer, pH 8.3/50% (v/v) DMSO, containing 100 mM Boc-Phe-OCam, 300 mM H-Leu-NH2 and 100 μM trypsinogen is agitated at room temperature under pH control. After 3 hours, the reaction is stopped as described in Example 3. The yield is analytically determined with HPLC to be 93% of theory.

Provenance & Evidence