Hydrochloric Acid → HCID 178508220, compound

Participants

Reaction Steps

To a solution of the above preparation of (cis)-3-[2-amino-6-(phenylmethoxy)-9H-purin-9-yl]-cyclobutanemethanol (330 mg) in 5.2 ml of methanol was added 2.6 ml of 3N HCl. The mixture was stirred under nitrogen at 40° for 2 hours and concentrated in vacuo to a solid, which was suspended in water. The pH was adjusted to 7.0 using 1N KOH, and the water was removed in vacuo leaving a solid. Chromatography of this solid on 80 ml of CHP-20P resin (Mitsubishi Chemical Industries) water and then 5% dimethylformamide in water gave 140 mg of (cis)-2-amino-1,9-dihydro-9-[3-(hydroxymethyl)cyclobutyl]-6H-purin-6-one as a solid having m.p. 275°-278° (dec.).

Provenance & Evidence