(2S,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate → Methyl 2-((benzoylamino)methyl)-3-oxobutanoate

Participants

Reaction Steps

The ADH-LK variant with SEQ ID No. 90 was used for further evolution of an efficient KRED for production of (2S,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate. Similarly, an ADH-LK variant with SEQ ID No.94 containing 10 mutations compared to ADH-LK which gave primarily the (2R,3R) stereoisomer, was used for further evolution of an efficient KRED for production of (2R,3R)-methyl-2-benzamidomethyl-3-hydroxybutyrate.

Provenance & Evidence