反应详情

温度

125 °C

A mixture of 37.5 mg of methyl 3-{[2-(difluoromethoxy)benzyl]oxy}-5-(3H-imidazo[4,5-c]pyridin-3-yl)thiophene-2-carboxylate and 5 ml of a saturated solution of ammonia in methanol is stirred in a microwave vial at 125° C. for 4 h in the microwave cavity. The reaction mixture is concentrated to dryness, the resulting residue is dissolved in a mixture of acetonitrile and methanol (9:1 (v/v)) and purified by preparative HPLC (water/acetonitrile, elution gradient 9/1 to 1/9 (v/v)) to obtain the title compound.

1. concentrationThe reaction mixture is concentrated to dryness
2. dissolutionthe resulting residue is dissolved in a mixture of acetonitrile and methanol (9:1 (v/v))
3. custompurified by preparative HPLC (water/acetonitrile, elution gradient 9/1 to 1/9 (v/v))