Phosphate → Trehalose

HRID 2393231Notes

Reaction equation

HRID 2393231 reaction equation
[CH2:1]([OH:23])[C@H:2]1[O:7][C@H:6]([O:8][C@H:9]2[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@H:12]([OH:17])O[C@@H]2CO)[C@H:5]([OH:20])[C@@H:4]([OH:21])[C@@H:3]1[OH:22].P([O-])([O-])([O-])=O>>[CH2:5]([OH:20])[C@H:6]1[O:7][C@H:9]([O:8][C@H:6]2[O:7][C@H:2]([CH2:1][OH:23])[C@@H:3]([OH:22])[C@H:4]([OH:21])[C@H:5]2[OH:20])[C@H:14]([OH:15])[C@@H:13]([OH:16])[C@@H:12]1[OH:17]

Participants

3

Reactant 2

HCID 1061PhosphateHCID 6255beta-Maltose

Product 1

HCID 7427Trehalose

Procedure

Five units each per 1 g of the substrates of purified extracellular trehalose phosphorylase and purified extracellular maltose phosphorylase produced in the same manners as in Examples 2 and 1 were added to 10 ml of 20% (W/V) solution of high maltose syrup (trade name; MC-95, Nihon Shokuhin Kako Co., Ltd, Sugar composition; 2.5% of glucose, 95.2% of maltose, 0.8% of maltotriose and 1.5% of maltotetraose) containing 5 mM phosphate buffer (pH 6.0) and allowed to react as in Example 6. Trehalose formed was measured by the HPLC method described above and it was found to correspond to 54.3% of the weight of the used substrates.

Workup

  1. customFive units each per 1 g of the substrates of purified extracellular trehalose phosphorylase and purified extracellular maltose phosphorylase
  2. customproduced in the same manners as in Examples 2 and 1
  3. customallowed to react as in Example 6

Sources & evidence

ORD record
ord-1efdca5be6384f108785784b0a9c67ee
Source dataset
uspto-grants-1998_09
Patent number
US05807719

Reaction notes

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