Lithium Chloride → Micronomicin

HRID 2395207Notes

Reaction equation

HRID 2395207 reaction equation
[Li+].[Cl-].[CH3:3][C@@:4]1([OH:34])[C@H:9]([NH:10][CH3:11])[C@@H:8]([OH:12])[C@@H:7]([O:13][C@@H:14]2[C@@H:19]([OH:20])[C@H:18]([O:21][C@H:22]3[O:27][C@H:26]([CH2:28][NH:29][CH3:30])[CH2:25][CH2:24][C@H:23]3[NH2:31])[C@@H:17]([NH2:32])[CH2:16][C@H:15]2[NH2:33])[O:6][CH2:5]1>O>[CH3:3][C@@:4]1([OH:34])[C@H:9]([NH:10][CH3:11])[C@@H:8]([OH:12])[C@@H:7]([O:13][C@@H:14]2[C@@H:19]([OH:20])[C@H:18]([O:21][C@H:22]3[O:27][C@H:26]([CH2:28][NH:29][CH3:30])[CH2:25][CH2:24][C@H:23]3[NH2:31])[C@@H:17]([NH2:32])[CH2:16][C@H:15]2[NH2:33])[O:6][CH2:5]1.[CH3:3][C:4]1([OH:34])[CH:9]([NH:10][CH3:11])[CH:8]([OH:12])[CH:7]([O:13][CH:14]2[CH:19]([OH:20])[CH:18]([O:21][CH:22]3[O:27][CH:26]([CH2:28][NH2:29])[CH2:25][CH2:24][CH:23]3[NH2:31])[CH:17]([NH2:32])[CH2:16][CH:15]2[NH2:33])[O:6][CH2:5]1

Participants

5

Reactant 2

HCID 433294Lithium ChlorideHCID 3037206Micronomicin

Product 2

HCID 582362HCID 582362, compoundYield 20%HCID 3037206MicronomicinYield 60%

Reagents, catalysts & solvents 1

HCID 962WaterSolvent2×

Procedure

10 ml sterilized water was added to one slant of M.echinospora JIM-401, and spores were scraped down and removed into a sterilized shake flask filled with glass balls. The flask was shaken for 15 min. The spore solution was filtered through sterilized filter paper to obtain monospora suspension. The suspension was diluted and treated with 0.3% LiCl at 30° C. for 30 min, then with UV (30 W) for 3 min. The treated suspension was diluted with sterilized water, spread on dishes containing 2000 ug/ml Micronomicin, and cultured at 35° C. for 14 days. Monocolonys were picked up and seeded on the slant, and were cultured at 37° C. for 8 days, then it was seeded in a shake flask, and shake-cultured at 35° C. for 6 days. The broth was detected with TLC and M.echinospora mutant CGMCC0197 was obtained. The mutant differed from original strain in cultural characteristics, physiologico-biochemical properties, carbon source utilization and products of fermentation. The mother strain produces Micronomicin (60%~70%) as the main component and gentamicin C1a (20%), but the mutant CGMCC0197 only produces a monocomponent antibiotic, which is the same as gentamicin C1a (GMC1)identified by means of TLC, UV, IR, NMR, MS.

Workup

  1. additionwas added to one slant of M.echinospora JIM-401, and spores
  2. customremoved into a sterilized shake flask
  3. additionfilled with glass balls
  4. filtrationThe spore solution was filtered through sterilized filter paper
  5. customto obtain monospora suspension
  6. additionThe suspension was diluted
  7. waitwith UV (30 W) for 3 min
  8. waitcultured at 35° C. for 14 days
  9. waitwere cultured at 37° C. for 8 days
  10. customit was seeded in a shake flask
  11. waitshake-cultured at 35° C. for 6 days
  12. customwas obtained

Sources & evidence

ORD record
ord-dfde9da2d6f644d1ab7ca8e19e60c20b
Source dataset
uspto-grants-1998_09
Patent number
US05814488

Reaction notes

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