Dimethyl Sulfoxide → HCID 6510267, compound

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Reaction Steps

All-trans retinol, all-trans retinal and all-trans retinoic acid were purchased from Sigma Chemical Co. (St. Louis, Mo.). The retinoids were dissolved at a concentration of 3×10-2M in methanol or DMSO with 10-4M butylated hydroxytoluene (Sigma Chemical Co.) added and stored in the dark at -20° C. in a nitrogen atmosphere. Immediately before use, the stock solutions were diluted in serum-free medium. 14-Hydroxy-retro-retinol (14HRR) was isolated from the pellets of HeLa cells fed with retinol by using a series of reversed-phase HPLC columns as described (33). 14HRR was pure according to the retention time on an analytical C18 reversed-phase column and the typical UV absorption spectrum.

Provenance & Evidence