Sodium Hydroxide → Acetate

Participants

Reaction Steps

To a solution of 4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine-5(6H)-propanoic acid, 8-[[4-(iminoaminomethyl)phenyl]methoxy]-1-methyl-6-oxo, ethyl ester in 3 mL ethanol at room temperature was added 0.75 mL 2N sodium hydroxide and the reaction stirred for 45 mins. The mixture was quenched 0.5 mL glacial acetic acid and purified by HPLC (solvent gradient 1:4 acetonitrile:water(0.5% acetic acid) (time 0 to 10 mins.) to 3:2 acetonitrile:water(0.5% acetic acid) (time 50 mins.), 20 mL/min, C18 2" column, Rf =33.2 mins.) to yield 4H-[1,2,4]triazolo[4,3-a][1,4]benzodiazepine-5(6H)-propanoic acid, 8-[[4-(iminoaminomethyl)phenyl]methoxy]-1-methyl-6-oxo, acetate. Exact mass (FAB, MH+) cald for C22H23N6O4 : 435.1781, found 435.1744.

Provenance & Evidence