Hydrochloric Acid → HCID 178468103, compound

Participants

Reaction Conditions

Reaction Steps

To a solution of 500 mg of Glu-His-Trp-NH-NH2 in 6 ml of dimethyl formamide were added, at -30° C, 0.66 ml of a 6.05 N HCl/dioxane solution and 1.2 ml of a 10% tert.-butyl nitrile solution in absolute dioxane. The solution was stirred for 20 minutes at -10° C and 877.8 mg of crude H-Ser-Tyr-D-Ser(But)-Leu-Arg-Pro-NH-C2H5 . 2 HCl and 0.78 ml of N-ethyl morpholine were added at -40° C. The mixture was allowed to stand overnight at 4° C. It was concentrated and the residue was triturated with ether. The substance was dissolved in water and chromatographed over Dowex(R) 1×2 (acetate form). The eluate was concentrated and purified over a carboxymethyl cellulose column (90 × 1.5 cm), that was equilibrated with 0.002 m NH4 -acetate solution. The substance was added as a solution in a 0.002 m NH4 -acetate solution. It was eluted with a 0.002 m NH4 -acetate solution in which the gradient of a 0.1 m NH4 -acetate solution was established (mixed volume 250 ml).

Provenance & Evidence