Mercapto-ethanol → HCID 178468104, compound

Participants

Reaction Steps

200 mg of Glu-His-Trp-Ser-Tyr-D-Ser(But)-Leu-Arg-Pro-NH-C2H5 -diacetate were dissolved with 0.1 ml of mercaptoethanol in 2 ml of trifluoroacetic acid. The solution was allowed to stand for 1 hour at room temperature, it was concentration and the residue was triturated with ether. The substance was suction-filtered and washed with ether, dissolved in water and chromatographed over Dowex 1×2 (acetate form). The eluate was concentrated and the residue was purified in analogy to Example 1 e) on Sephadex LH 20. Yield: 120 mg. The content of peptide base was 77.5% as per UV-spectrum [-α]D20 = -43.7° (c=1, H2O).

Provenance & Evidence