HCID 46223008, compound → Raffinose

Participants

Reaction Steps

A solution of 2.0 g of D-raffinose pentahydrate in 50 ml of absolute dimethylformamide was stirred at 60° C. for 20 hours in the presence of 10.3 g of sulphur trioxide-trimethylamine complex, whereby a precipitate separated. The solvent was decanted off. The residue was washed with methanol, dissolved in 64 ml of aqueous 10% sodium acetate solution and concentrated. The residue was taken up several times in water and evaporated to remove trimethylamine. The residue was gel-chromatographed (Sephadex® LH 20) in order to remove salts. After freeze-drying there were obtained 5.28 g of sulfated raffinose, S=19.89%, AS (average degree of sulfation) about 2.8.

Provenance & Evidence