Sodium Hydroxide → HCID 67193350, compound

Participants

Reaction Steps

To a solution of 4-[(8-cyclopentyl-6-oxo-1-propyl-6,7-dihydro-1H-purin-2-ylamino)-methyl]-benzoic acid methyl ester (0.081 g, 0.198 mmol) in a mixture of MeOH/H2O/THF (5 ml each) was added NaOH (0.024 g, 0.594 mmol) and the resulting reaction mixture was refluxed overnight. The reaction mixture was concentrated under reduced pressure. The residue was dissolved in water and neutralized with 1N HCl and then extracted with ethyl acetate. The ethyl acetate layer was dried over anhydrous sodium sulphate and concentrated under vacuum. The crude product was purified by washing repeatedly with diethyl ether to give the product (0.025 g, 32%) as an off white solid.

Provenance & Evidence