Reaction Steps
A mixture of methyl 4-((1S,3aS,5aR,5bR,7aR,11aS,11bR,13aR,13bR)-3a-(2-(dimethylamino)ethylcarbamoyl)-1-isopropyl-5a,5b,8,8,11a-pentamethyl-2,3,3a,4,5,5a,5b,6,7,7a,8,11,11a,11b,12,13,13a,13b-octadecahydro-1H-cyclopenta[a]chrysen-9-yl)benzoate (6 mg, 9.30 mmol) and sodium hydroxide (0.037 mL, 0.037 mmol) in dioxane (1 mL) was heated for 3 hours at 78° C. The reaction mixture was filtered and purified by prep HPLC to provide the title compound as a white solid (2.2 mg, 36%). LCMS: m/e 631.59 (M+H)+, 2.29 min (method 1). 1H NMR (500 MHz, Acetic Acid-d4) δ ppm 8.03 (d, J=8.24 Hz, 2H), 7.30 (d, J=8.24 Hz, 2H), 5.38 (d, J=4.58 Hz, 1H), 3.74 (t, J=6.10 Hz, 2H), 3.46-3.33 (m, 2H), 2.96 (s, 6H), 2.63-2.47 (m, 1H), 2.44-2.27 (m, 1H), 2.25-1.15 (m, 22H), 1.08 (s, 3H), 1.06 (s, 3H), 1.05 (s, 3H), 1.01 (s, 3H), 1.00 (s, 3H), 0.91 (d, J=6.71 Hz, 3H), 0.82 (d, J=6.71 Hz, 3H).
