反应详情

cis-9-Myristoleate, 3 (4.2 g, 0.0175 mol) was enzymatically transesterified with cetyl alcohol (5.08 g, 0.021 mol) in the presence of Novozyme 435 (0.930 g, 10 wt % of the total substrate) at 68° C. for 8 hr. The reaction was monitored by TLC and after completion of the reaction, hexane (50 ml) was added and the lipase was separated by filtration and the solvent was evaporated to get the crude product and was purified by column chromatography to obtain hexadecyl cis-9-tetradecenoate, 4 (7.48 g, 0.0166 mol) in 90% yield with 90% purity by GC. The structure of hexadecyl cis-9-tetradecenoate, 4 was confirmed by 1H NMR, IR, and GC-MS.

1. customat 68° C.
2. customfor 8 hr
3. additionwas added
4. customthe lipase was separated by filtration
5. customthe solvent was evaporated
6. customto get the crude product
7. customwas purified by column chromatography